Metabolic survey of defense responses to a compatible hemibiotroph, Phytophthora parasitica var. nicotianae, in ethylene signaling-impaired tobacco.
Identifieur interne : 001272 ( Main/Exploration ); précédent : 001271; suivant : 001273Metabolic survey of defense responses to a compatible hemibiotroph, Phytophthora parasitica var. nicotianae, in ethylene signaling-impaired tobacco.
Auteurs : Kyoungwon Cho [Corée du Sud] ; Yuran Kim ; Soo Jin Wi ; Jong Bok Seo ; Joseph Kwon ; Joo Hee Chung ; Ky Young Park ; Myung Hee NamSource :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2013.
Descripteurs français
- KwdFr :
- Espèces réactives de l'oxygène (métabolisme), Maladies des plantes (parasitologie), Phytophthora (MeSH), Protéines végétales (génétique), Résistance à la maladie (MeSH), Tabac (génétique), Tabac (métabolisme), Tabac (parasitologie), Transduction du signal (génétique), Végétaux génétiquement modifiés (métabolisme), Végétaux génétiquement modifiés (parasitologie), Éthylènes (MeSH).
- MESH :
- génétique : Protéines végétales, Tabac, Transduction du signal.
- métabolisme : Espèces réactives de l'oxygène, Tabac, Végétaux génétiquement modifiés.
- parasitologie : Maladies des plantes, Tabac, Végétaux génétiquement modifiés.
- Phytophthora, Résistance à la maladie, Éthylènes.
English descriptors
- KwdEn :
- Disease Resistance (MeSH), Ethylenes (MeSH), Phytophthora (MeSH), Plant Diseases (parasitology), Plant Proteins (genetics), Plants, Genetically Modified (metabolism), Plants, Genetically Modified (parasitology), Reactive Oxygen Species (metabolism), Signal Transduction (genetics), Tobacco (genetics), Tobacco (metabolism), Tobacco (parasitology).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Reactive Oxygen Species.
- chemical : Ethylenes.
- genetics : Signal Transduction, Tobacco.
- metabolism : Plants, Genetically Modified, Tobacco.
- parasitology : Plant Diseases, Plants, Genetically Modified, Tobacco.
- Disease Resistance, Phytophthora.
Abstract
Reactive oxygen species (ROS) and ethylene play an important role in determining the resistance or susceptibility of plants to pathogen attack. A previous study of the response of tobacco cultivar ( Nicotiana tabacum L. cv. Wisconsin 38) to a compatible hemibiotroph, Phytophthora parasitica var. nicotianae (Ppn) showed that biphasic bursts of ROS and ethylene are positively associated with disease severity. The levels of ethylene and ROS might influence the susceptibility of plants to pathogens, with changing levels of metabolite related to disease resistance or susceptibility. In this study, to obtain more detailed information on the interaction of ROS and ethylene signaling related to resistance and/or susceptibility of plants to pathogen, Ppn-induced metabolic profiles from wild type (WT) and ethylene signaling-impaired transgenic plants that expressed Ein3 antisense (Ein3-AS) were compared using ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Nonredundant mass ions (576 in ESI+ mode and 336 in ESI- mode) were selected, and 56 mass ions were identified on the basis of their accurate mass ions and MS/MS spectra. Two-way hierarchical clustering analysis of the selected mass ions revealed that nicotine and phenylpropanoid-polyamine conjugates, such as caffeoyl-dihydrocaffeoyl-spermidine, dicaffeoyl-spermidine, caffeoyl-feruloyl-spermidine, and two bis(dihydrocaffeoyl)-spermine isomers, and their intermediates, such as arginine and putrecine, were present at lower levels in Ein3-AS transgenic plants during Ppn interaction than in WT, whereas galactolipid and oxidized free fatty acid levels were higher in Ein3-AS transgenic plants. Taken together, these results reveal a function for ethylene signaling in tobacco defense responses during Ppn interaction.
DOI: 10.1021/jf401785w
PubMed: 23866065
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Kim, Yuran" sort="Kim, Yuran" uniqKey="Kim Y" first="Yuran" last="Kim">Yuran Kim</name></author><author><name sortKey="Wi, Soo Jin" sort="Wi, Soo Jin" uniqKey="Wi S" first="Soo Jin" last="Wi">Soo Jin Wi</name></author><author><name sortKey="Seo, Jong Bok" sort="Seo, Jong Bok" uniqKey="Seo J" first="Jong Bok" last="Seo">Jong Bok Seo</name></author><author><name sortKey="Kwon, Joseph" sort="Kwon, Joseph" uniqKey="Kwon J" first="Joseph" last="Kwon">Joseph Kwon</name></author><author><name sortKey="Chung, Joo Hee" sort="Chung, Joo Hee" uniqKey="Chung J" first="Joo Hee" last="Chung">Joo Hee Chung</name></author><author><name sortKey="Park, Ky Young" sort="Park, Ky Young" uniqKey="Park K" first="Ky Young" last="Park">Ky Young Park</name></author><author><name sortKey="Nam, Myung Hee" sort="Nam, Myung Hee" uniqKey="Nam M" first="Myung Hee" last="Nam">Myung Hee Nam</name></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="eISSN">1520-5118</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Disease Resistance (MeSH)</term><term>Ethylenes (MeSH)</term><term>Phytophthora (MeSH)</term><term>Plant Diseases (parasitology)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Plants, Genetically Modified (parasitology)</term><term>Reactive Oxygen Species (metabolism)</term><term>Signal Transduction (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term><term>Tobacco (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Maladies des plantes (parasitologie)</term><term>Phytophthora (MeSH)</term><term>Protéines végétales (génétique)</term><term>Résistance à la maladie (MeSH)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Tabac (parasitologie)</term><term>Transduction du signal (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term><term>Végétaux génétiquement modifiés (parasitologie)</term><term>Éthylènes (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Ethylenes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Signal Transduction</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines végétales</term><term>Tabac</term><term>Transduction du signal</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Espèces réactives de l'oxygène</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Resistance</term><term>Phytophthora</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Phytophthora</term><term>Résistance à la maladie</term><term>Éthylènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Reactive oxygen species (ROS) and ethylene play an important role in determining the resistance or susceptibility of plants to pathogen attack. A previous study of the response of tobacco cultivar ( Nicotiana tabacum L. cv. Wisconsin 38) to a compatible hemibiotroph, Phytophthora parasitica var. nicotianae (Ppn) showed that biphasic bursts of ROS and ethylene are positively associated with disease severity. The levels of ethylene and ROS might influence the susceptibility of plants to pathogens, with changing levels of metabolite related to disease resistance or susceptibility. In this study, to obtain more detailed information on the interaction of ROS and ethylene signaling related to resistance and/or susceptibility of plants to pathogen, Ppn-induced metabolic profiles from wild type (WT) and ethylene signaling-impaired transgenic plants that expressed Ein3 antisense (Ein3-AS) were compared using ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Nonredundant mass ions (576 in ESI+ mode and 336 in ESI- mode) were selected, and 56 mass ions were identified on the basis of their accurate mass ions and MS/MS spectra. Two-way hierarchical clustering analysis of the selected mass ions revealed that nicotine and phenylpropanoid-polyamine conjugates, such as caffeoyl-dihydrocaffeoyl-spermidine, dicaffeoyl-spermidine, caffeoyl-feruloyl-spermidine, and two bis(dihydrocaffeoyl)-spermine isomers, and their intermediates, such as arginine and putrecine, were present at lower levels in Ein3-AS transgenic plants during Ppn interaction than in WT, whereas galactolipid and oxidized free fatty acid levels were higher in Ein3-AS transgenic plants. Taken together, these results reveal a function for ethylene signaling in tobacco defense responses during Ppn interaction. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23866065</PMID><DateCompleted><Year>2014</Year><Month>07</Month><Day>04</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5118</ISSN><JournalIssue CitedMedium="Internet"><Volume>61</Volume><Issue>35</Issue><PubDate><Year>2013</Year><Month>Sep</Month><Day>04</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J Agric Food Chem</ISOAbbreviation></Journal><ArticleTitle>Metabolic survey of defense responses to a compatible hemibiotroph, Phytophthora parasitica var. nicotianae, in ethylene signaling-impaired tobacco.</ArticleTitle><Pagination><MedlinePgn>8477-89</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/jf401785w</ELocationID><Abstract><AbstractText>Reactive oxygen species (ROS) and ethylene play an important role in determining the resistance or susceptibility of plants to pathogen attack. A previous study of the response of tobacco cultivar ( Nicotiana tabacum L. cv. Wisconsin 38) to a compatible hemibiotroph, Phytophthora parasitica var. nicotianae (Ppn) showed that biphasic bursts of ROS and ethylene are positively associated with disease severity. The levels of ethylene and ROS might influence the susceptibility of plants to pathogens, with changing levels of metabolite related to disease resistance or susceptibility. In this study, to obtain more detailed information on the interaction of ROS and ethylene signaling related to resistance and/or susceptibility of plants to pathogen, Ppn-induced metabolic profiles from wild type (WT) and ethylene signaling-impaired transgenic plants that expressed Ein3 antisense (Ein3-AS) were compared using ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Nonredundant mass ions (576 in ESI+ mode and 336 in ESI- mode) were selected, and 56 mass ions were identified on the basis of their accurate mass ions and MS/MS spectra. Two-way hierarchical clustering analysis of the selected mass ions revealed that nicotine and phenylpropanoid-polyamine conjugates, such as caffeoyl-dihydrocaffeoyl-spermidine, dicaffeoyl-spermidine, caffeoyl-feruloyl-spermidine, and two bis(dihydrocaffeoyl)-spermine isomers, and their intermediates, such as arginine and putrecine, were present at lower levels in Ein3-AS transgenic plants during Ppn interaction than in WT, whereas galactolipid and oxidized free fatty acid levels were higher in Ein3-AS transgenic plants. Taken together, these results reveal a function for ethylene signaling in tobacco defense responses during Ppn interaction. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cho</LastName><ForeName>Kyoungwon</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Seoul Center, Korea Basic Science Institute (KBSI) , Seoul 136-713, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Yuran</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Wi</LastName><ForeName>Soo jin</ForeName><Initials>Sj</Initials></Author><Author ValidYN="Y"><LastName>Seo</LastName><ForeName>Jong Bok</ForeName><Initials>JB</Initials></Author><Author ValidYN="Y"><LastName>Kwon</LastName><ForeName>Joseph</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Chung</LastName><ForeName>Joo Hee</ForeName><Initials>JH</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Ky Young</ForeName><Initials>KY</Initials></Author><Author ValidYN="Y"><LastName>Nam</LastName><ForeName>Myung Hee</ForeName><Initials>MH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>08</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Agric Food Chem</MedlineTA><NlmUniqueID>0374755</NlmUniqueID><ISSNLinking>0021-8561</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005030">Ethylenes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>91GW059KN7</RegistryNumber><NameOfSubstance UI="C036216">ethylene</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005030" MajorTopicYN="Y">Ethylenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="Y">Phytophthora</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="Y">Signal Transduction</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>7</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>7</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23866065</ArticleId><ArticleId IdType="doi">10.1021/jf401785w</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><noCountry><name sortKey="Chung, Joo Hee" sort="Chung, Joo Hee" uniqKey="Chung J" first="Joo Hee" last="Chung">Joo Hee Chung</name><name sortKey="Kim, Yuran" sort="Kim, Yuran" uniqKey="Kim Y" first="Yuran" last="Kim">Yuran Kim</name><name sortKey="Kwon, Joseph" sort="Kwon, Joseph" uniqKey="Kwon J" first="Joseph" last="Kwon">Joseph Kwon</name><name sortKey="Nam, Myung Hee" sort="Nam, Myung Hee" uniqKey="Nam M" first="Myung Hee" last="Nam">Myung Hee Nam</name><name sortKey="Park, Ky Young" sort="Park, Ky Young" uniqKey="Park K" first="Ky Young" last="Park">Ky Young Park</name><name sortKey="Seo, Jong Bok" sort="Seo, Jong Bok" uniqKey="Seo J" first="Jong Bok" last="Seo">Jong Bok Seo</name><name sortKey="Wi, Soo Jin" sort="Wi, Soo Jin" uniqKey="Wi S" first="Soo Jin" last="Wi">Soo Jin Wi</name></noCountry><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Cho, Kyoungwon" sort="Cho, Kyoungwon" uniqKey="Cho K" first="Kyoungwon" last="Cho">Kyoungwon Cho</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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